| 货号 | 8475S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | YB1 (D2B12) Rabbit mAb recognizes endogenous levels of total YB1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | The Y-box binding protein 1 (YB1) belongs to a family of evolutionarily conserved, multifunctional Y-box proteins that bind single-stranded DNA and RNA and function as regulators of transcription, RNA metabolism, and protein synthesis (1). YB1 binds to Y-box sequences (TAACC) found in multiple gene promoters and can positively or negatively regulate transcription. YB1 activates genes associated with proliferation and cancer, such as cyclin A, cyclin B1, matrix metalloproteinase-2 (MMP-2), and the multi-drug resistance 1 (MDR1) gene (2-4). YB1 represses genes associated with cell death, including the Fas cell death-associated receptor and the p53 tumor suppressor gene (5-7). It also interacts with the RNA-splicing factor SRp30c and stabilizes interleukin-2 (IL-2) mRNA upon induction of T lymphocytes by IL-2 (8,9). The majority of YB1 protein localizes to the cytoplasm, with a minor pool found in the nucleus; however, nuclear localization appears to be critical for its role in promoting proliferation. Nuclear translocation is cell cycle regulated, with YB1 protein accumulating in the nucleus during G1/S phase (2). In addition, nuclear translocation is induced in response to extracellular stimuli such as hyperthermia and UV irradiation, or treatment of cells with thrombin, interferons, or insulin-like growth factor (IGF-I) (2,10). Treatment of the MCF7 breast cancer cell line with IGF-I results in Akt-mediated phosphorylation of YB1 at Ser102, which is required for nuclear translocation of YB1 and its ability to promote anchorage-independent growth (10). Research studies have shown that YB1 is overexpressed in many malignant tissues, including breast cancer, non-small cell lung carcinoma, ovarian adenocarcinomas, human osteosarcomas, colorectal carcinomas, and malignant melanomas. Investigators have shown that nuclear YB1 expression correlates with high levels of proliferation, drug resistance, and poor tumor prognosis (2,7,10). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using YB1 (D2B12) Rabbit mAb. 免疫组化分析石蜡包埋的人类乳腺癌细胞，所用抗体为YB1 (D2B12) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from various cell lines using YB1 (D2B12) Rabbit mAb. western blot 分析不同细胞系提取物，所用抗体为YB1 (D2B12) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using YB1 (D2B12) Rabbit mAb. 免疫组化分析石蜡包埋的人类结肠癌细胞，所用抗体为YB1 (D2B12) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma using YB1 (D2B12) Rabbit mAb. 免疫组化分析石蜡包埋的人类肺脏鳞状细胞癌，所用抗体为YB1 (D2B12) Rabbit mAb兔单抗。 |
