| 货号 | 12395S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | YAP (1A12) Mouse mAb recognizes endogenous levels of total YAP protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 65-75 |
Immunohistochemical analysis of paraffin-embedded cell pellets, PANC-1 (left) or Jurkat (right), using YAP (1A12) Mouse mAb. 使用YAP (1A12)Mouse mAb鼠单抗对石蜡包埋的细胞，PANC-1（左）或Jurkat细胞（右）进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using YAP (1A12) Mouse mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). 使用YAP (1A12)Mouse mAb鼠单抗（上）和β-actin (D6A8) Rabbit mAb 兔单抗#8457（下）对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded breast carcinoma using YAP (1A12) Mouse mAb. 使用YAP (1A12)Mouse mAb鼠单抗对石蜡包埋的乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma using YAP (1A12) Mouse mAb. 使用YAP (1A12)Mouse mAb鼠单抗对石蜡包埋的卵巢癌组织进行免疫组化分析。 |
