XPB (2C6) Mouse mAb
货号：
8746S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 8746S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 使用方法 | WB(1:1000)
IP (1:50) |
性能| 供应商 | CST |
| 背景 | XPB and XPD are ATPase/helicase subunits of the TFIIH complex that are involved in nucleotide excision repair (NER) to remove lesions and photoproducts generated by UV light (1). XPB and XPD are 3’-5’ and 5’-3’ DNA helicases, respectively, that play a role in opening of the DNA damage site to facilitate repair (2,3). XPB and XPD both play an important role in maintaining genomic stability, and mutations of these proteins cause Xeroderma Pigmentosum (XP) and Trichothiodystrophy (TTD). XP patients have abnormalities in skin pigmentation and are highly susceptible to skin cancers, while TTD patients exhibit symptoms such as brittle hair, neurological abnormalities, and mild photosensitivity (4). In addition to their role in NER, XPB and XPD are involved in transcription initiation as part of the TFIIH core complex (5). The helicase activity of XPB unwinds DNA around the transcription start site to facilitate RNA polymerase II promoter clearance and initiation of transcription (6). XPD plays a structural role linking core TFIIH components with the cdk-activating kinase (CAK) complex that phosphorylates the C-terminus of the largest subunit of RNA polymerase II, leading to transcription initiation (7).XPB和XPD是TFIIH复合体的ATP酶/解旋酶亚基，参与核苷酸切除修复（NER）以清除损伤和紫外产生的磷酸化产物（1）。XPB和XPD分别是3-5和5-3DNA解旋酶，在打开DNA损伤部位以便进行维修复的过程中发挥作用（2,3）。 XPB和XPD在维护基因组的稳定性以及这些蛋白质的基因突变造成着色性干皮病（XP）和毛发硫营养不良(（TTD）的过程中发挥了重要的作用。XP病人有异常的皮肤色素沉着，并具有很高的皮肤癌易感性，而TTD患者表现出其它的症状，如发脆，神经系统异常，轻度的光敏性（4）。除了它们在NER中的作用，XPB和XPD作为TFIIH核心复合物的一部分参与转录起始（5）。XPB的解旋酶活性能够在转录起始位点周围解旋DNA，促进RNA聚合酶II启动子清除和转录起始（6）。 XPD通过连接核心TFIIH组件与cdk活化激酶（CAK）复合物来发挥结构功能，磷酸化RNA聚合酶II最大亚基的C-末端，导致转录起始（7）。 |
| 存放说明 | -20C |
| 计算分子量 | 89 |
参考图片Western blot analysis of extracts from various cell lines using XPB (2C6) Mouse mAb.Western blot 方法检测不同细胞提取物，使用的抗体为 XPB (2C6) Mouse mAb。 |
