| 货号 | 13084S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:400) |
| 供应商 | CST |
| 背景 | Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Flow cytometric analysis of Jurkat cells using Wee1 (D10D2) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.使用Wee1 (D10D2) Rabbit mAb兔单抗和碘化丙啶(PI)/RNase Staining Solution #4087处理Jurkat细胞然后进行流式分析以确定DNA量。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #441被用作二抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Wee1 (D10D2) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照肽段(左)和抗原特异性肽段(右)存在的前提下，使用Wee1 (D10D2) Rabbit mAb对石蜡包埋的人结肠癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded HT-29 (left) or PC-3 (right) cell pellets using Wee1 (D10D2) Rabbit mAb.未处理(-)或使用格尔德霉素#9843 (+)处理的HeLa细胞提取物，使用Wee1 (D10D2) Rabbit mAb (上) 和 β-Actin (D6A8) Rabbit mAb #8457 (下)进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Wee1 (D10D2) Rabbit mAb兔单抗.使用Wee1 (D10D2) Rabbit mAb对石蜡包埋的HT-29(左)或PC-3(右)进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using Wee1 (D10D2) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb兔单抗#8457 (lower).使用Wee1 (D10D2) Rabbit mAb兔单抗(上) 和β-Actin (D6A8) Rabbit mAb兔单抗#8457 (下).对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Geldanamycin #9843 (+), using Wee1 (D10D2) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).使用Wee1 (D10D2) Rabbit mAb兔单抗进行Western blot分析。 |
