| 货号 | 14764S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Vesicle transport through interaction with t-SNAREs homolog 1 (Vti1) has two protein members, Vti1a and Vti1b. Human Vti1 was first identified as a homolog of the yeast v-SNARE Vti1p and was able to functionally rescue the phenotype of Vti1p-deficient yeast (1). The mammalian proteins Vti1a and Vti1b exhibit distinct but overlapping localization. Vti1a and Vti1b are both localized in the trans-Golgi network, with Vti1a also found in the Golgi apparatus and Vti1b in endosomes (2). Vti1 proteins have been implicated in a number of protein-protein interactions with partners such as VAMP4, syntaxin 6, syntaxin 8, syntaxin 16, and synaptobrevin (2-4). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Vti1a protein (hVti1a-Myc; +), using Vti1a (D8U3M) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines and tissues using Vti1a (D8U3M) Rabbit mAb. | |
Immunoprecipitation of Vti1a from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Vti1a (D8U3M) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Vti1a (D8U3M) Rabbit mAb. | |
Confocal immunofluorescent analysis of NIH/3T3 (left) and A-204 (right) cells using Vti1a (D8U3M) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Pseudocolor blue = DRAQ5® #4084 (fluorescent DNA dye). |
