| 货号 | 9698S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F/F |
| 目标/特异性 | VEGF Receptor 2 (D5B1) Rabbit mAb recognizes endogenous levels of total VEGF receptor 2 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:1600) F (1:400) IF-F (1:400) |
| 供应商 | CST |
| 背景 | Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 210, 230 |
Western blot analysis of extracts from HUVE cells, HeLa cells and mouse heart using VEGF Receptor 2 (D5B1) Rabbit mAb for VEGFR2 expression (upper) and β-Actin (D6A8) Rabbit mAb (#8457) for loading control. (The 80 kDa bands represent partial degradation product).Western blot方法检测HUVE细胞， HeLa 细胞核小鼠心脏组织提取物，使用的抗体为：VEGF Receptor 2 (D5B1) Rabbit mAb 用于检测 VEGFR2 表达(上图)，β-Actin (D6A8) Rabbit mAb (#8457)用作内参。（80kDa条带代表产物部分降解）。 | |
Confocal immunofluorescent analysis of rat pancreas using VEGF Receptor 2 (D5B1) Rabbit mAb (green) and β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光法检测大鼠胰腺组织，使用的抗体为VEGF Receptor 2 (D5B1) Rabbit mAb (绿色)和β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (红色)。蓝色伪彩色为DNA荧光染料，产品信息为DRAQ5® #4084。 | |
Immunohistochemical analysis of paraffin-embedded human angiosarcoma using VEGF Receptor 2 (D5B1) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人血管肉瘤组织，使用的抗体为VEGF Receptor 2 (D5B1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse liver using VEGF Receptor 2 (D5B1) Rabbit mAb.免疫组织化学方法检测石蜡包埋的小鼠肝组织，使用的抗体为VEGF Receptor 2 (D5B1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded HCC827 xenograft using VEGFR2 (D5B1) Rabbit mAb.免疫组织化学方法检测石蜡包埋的HCC827移植瘤组织，使用的抗体为VEGFR2 (D5B1) Rabbit mAb. | |
Flow cytometric analysis of HeLa cells (blue) and HUVEC cells (green) using VEGF Receptor 2 (D5B1) Rabbit mAb.流式细胞术方法分析HeLa细胞(蓝色)和HUVEC细胞（绿色），使用的抗体为VEGF Receptor 2 (D5B1) Rabbit mAb. |
