| 货号 | 13508S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:3200) IF-F (1:2000) IF-IC (1:250) |
| 供应商 | CST |
| 背景 | Vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin) is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in vesicular transport and membrane fusion, a process regulated by calcium (2). In neurons, VAMP2 is predominantly inserted in presynaptic vesicle membranes. Assembly of VAMP2 with the plasma membrane SNAREs syntaxin 1 and SNAP25 is a key event necessary for membrane fusion and neurotransmitter release (2). In addition to this important function, VAMP2 is also involved in granule exocytosis in neutrophils (3) and release of bioactive peptides from cardiac myocytes (4) and juxtaglomerular cells (5). |
| 存放说明 | -20C |
| 计算分子量 | 13 |
Confocal immunofluorescent analysis of normal rat retina using VAMP2 (D6O1A) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines and tissues using VAMP2 (D6O1A) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human VAMP1 (hVAMP1-Myc; +) or VAMP2 (hVAMP2-Myc; +), using VAMP2 (D6O1A) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Immunoprecipitation of VAMP2 from Neuro-2a cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or VAMP2 (D6O1A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VAMP2 (D6O1A) Rabbit mAb. | |
Confocal immunofluorescent analysis of PC-12 cells using VAMP2 (D6O1A) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded rat kidney using VAMP2 (D6O1A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse pancreas using VAMP2 (D6O1A) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon using VAMP2 (D6O1A) Rabbit mAb. |
