| 货号 | 13115S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | UVRAG (D2Q1Z) Rabbit mAb recognizes endogenous levels of total UVRAG protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. These proteins are involved in the formation of cytoplasmic vacuoles called autophagosomes that are delivered to lysosomes for degradation. The class III type phosphoinositide 3-kinase (PI3KC3)/Vps34 regulates vacuolar trafficking as well as autophagy (4,5). Multiple proteins have been shown to be associated with Vsp34, including: p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon, which can determine Vsp34 function (6-11). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (11). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (12). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (13,14). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from various cell lines using UVRAG (D2Q1Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). HCT 116 cells have been reported to contain a monoallelic frameshift mutation resulting in significantly reduced levels of endogenous UVRAG expression (11). Western blot 分析多种细胞系的细胞提取物，使用抗体是UVRAG (D2Q1Z) Rabbit mAb 兔单抗 (上图) 或 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下图)。据报道，HCT116细胞含有一个单等位基因的移码突变，导致其显著诱导内源性的UVRAG的表达(11)。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human UVRAG (hUVRAG; +), using UVRAG (D2Q1Z) Rabbit mAb. Western blot 分析293T细胞的细胞提取物，对照转染(-)或转染过表达人源UVRAG 的质粒(hUVRAG; +)，使用抗体是 UVRAG (D2Q1Z) Rabbit mAb 兔单抗。 | |
Immunoprecipitation of UVRAG from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or UVRAG (D2Q1Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UVRAG (D2Q1Z) Rabbit mAb. 免疫共沉淀分析PANC-1细胞提取物中的UVRAG，使用抗体是using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 UVRAG (D2Q1Z) Rabbit mAb (泳道3). 泳道1是 10% input。Western blot分析所使用的抗体是UVRAG (D2Q1Z) Rabbit mAb 兔单抗。 |
