| 货号 | 8501S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | USP10 (D7A5) Rabbit mAb recognizes endogenous levels of total USP10 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP10 possesses amino acid sequences that match the consensus cysteine and histidine boxes representative of the USP family of deubiquitinating enzymes. At the posttranslational level, USP10 appears to be regulated through both protein-protein interactions and phosphorylation. Indeed, interaction of USP10 with Ras-GAP SH3 domain binding protein (G3BP) has been found to inhibit its ability to catalyze the disassembly of ubiquitin chains (3). Furthermore, ATM-mediated phosphorylation of USP10 at Thr42 and Ser337 was shown to promote USP10 stabilization and redistribution from the cytoplasm to the nucleus, where it functions in p53 deubiquitination, stabilization, and activation in response to genotoxic stress (4). Recently, it was shown that USP10 works in concert with USP13 and Vps34 complexes. USP10, along with USP13, appears to deubiquitinate Vps34 complexes to regulate the levels of this class III PI3K. Beclin-1, another component of these complexes, functions to regulate the stability of USP13, which can deubiquitinate and stabilize the levels of USP10. Therefore, Beclin-1, can indirectly regulate p53 stability by controlling the DUB activity of USP10 (5). USP10 also functions in the endosomal compartment, where it has been shown to deubiquitinate CFTR in order to enhance its endocytic recycling and cell surface expression (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Confocal immunofluorescent analysis of HCT 116 cells using USP10 (D7A5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).Confocal immunofluorescent检测HCT 116细胞，采用USP10 (D7A5) Rabbit mAb (绿色)。蓝色pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from various cell lines using USP10 (D7A5) Rabbit mAb. Western blot检测多种细胞提取物，采用USP10 (D7A5) Rabbit mAb。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP10 (hUSP10-Myc/DDK, +), using USP10 (D7A5) Rabbit mAb.Western blot采用检测mock transfected (-)或转染表达编码人全长USP10的Myc/DDK-tagged cDNA的293T细胞，采用USP10 (D7A5) Rabbit mAb。 |
