| 货号 | 68522S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP |
| 目标/特异性 | CIRBP (D1M8D) Rabbit mAb recognizes endogenous levels of total CIRBP protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cold-induced RNA-binding protein (CIRBP) is a 172-residue, multifunctional sensor protein that was first isolated as a protein induced in mouse fibroblasts cultured at 32ºC (1). Conversely, CIRBP expression decreases in cells or tissues subjected to increased temperature (2). The CIRBP protein is composed of an amino-terminal RNA-binding domain and a carboxyl-terminal, glycine-rich domain (1). Stressful stimuli, such as hypoxia, heat shock, osmotic shock, or oxidative conditions, lead to translocation of CIRBP from the nucleus to cytoplasmic stress granules through a mechanism involving CIRBP methylation-dependent nuclear export (3). CIRBP plays a role in regulating apoptosis and preserving the stemness of neural stem cells at moderately low temperatures (4). Research studies demonstrate that CIRBP contributes to the regulation of circadian rhythm through post-translational modulation of CLOCK expression (5). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . Nishiyama, H. et al. (1997) J Cell Biol 137, 899-908. 2 . Nishiyama, H. et al. (1998) Am J Pathol 152, 289-96. 3 . De Leeuw, F. et al. (2007) Exp Cell Res 313, 4130-44. 4 . Saito, K. et al. (2010) Brain Res 1358, 20-9. 5 . Morf, J. et al. (2012) Science 338, 379-83. |
Western blot analysis of 293T, SK-MEL-28, and SH-SY5Y cells, and human thyroid tissue extracts, using CIRBP (D1M8D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
