| 货号 | 67489S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | WB,IF |
| 目标/特异性 | c-Rel (D3B8S) Rabbit mAb recognizes endogenous levels of total c-Rel protein. |
| 使用方法 | Western Blotting (1:1000) Immunofluorescence (Immunocytochemistry) (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). c-Rel contains an amino-terminal DNA-binding domain referred to as the REL homology domain (RHD) and carboxy-terminal transactivation domains. The c-Rel protein is typically inhibited in unstimulated cells by IκBα and IκBβ. c-Rel expression is highest in hematopoietic cells with extensive research studies demonstrating its role in immune cell function and pathogenesis of disease (12,13). |
| 存放说明 | -20C |
| 计算分子量 | 68-78 |
| 参考文献 | 1 . Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2 . Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3 . Haskill, S. et al. (1991) Cell 65, 1281-9. 4 . Thompson, J.E. et al. (1995) Cell 80, 573-82. 5 . Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6 . Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7 . Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8 . Chen, Z.J. et al. (1996) Cell 84, 853-62. 9 . Senftleben, U. et al. (2001) Science 293, 1495-9. 10 . Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11 . Xiao, G. et al. (2001) Mol Cell 7, 401-9. 12 . Gilmore, T.D. and Gerondakis, S. (2011) Genes Cancer 2, 695-711. 13 . Fullard, N. et al. (2012) Int J Biochem Cell Biol 44, 851-60. |
Western blot analysis of extracts from various cell lines using c-Rel (D3B8S) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of RT4 cells, untreated (left) or treated with Tumor Necrosis Factor-α #2169 (20 ng/ml, 20 min; right), using c-Rel (D3B8S) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). |
