| 货号 | 92345S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP,IF |
| 目标/特异性 | TRAP1/HSP75 (D3D7N) Rabbit mAb recognizes endogenous levels of total TRAP1/HSP75 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) Immunofluorescence (Immunocytochemistry) (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TNF receptor-associated protein 1 (TRAP1), also known as HSP75, is a mitochondrial chaperone and ATPase that was originally identified as a protein that interacts with the TNF receptor. Although a member of the HSP90 family, TRAP1 is not heat-inducible but is upregulated by glucose deprivation, oxidative injury, and UV irradiation. An amino-terminal mitochondrial localization sequence results in localization of TRAP1 within mitochondria (1). Overexpression of TRAP1 decreases oxidative stress, suggesting a protective role in ischemia injury (2). Research studies demonstrate that silencing of TRAP1 enhances cytochrome C release and apoptosis, with additional evidence indicating that TRAP1 can protect cells from cell death by inhibiting the generation of reactive oxygen species (3). TRAP1 is a substrate of the mitochondrial serine/threonine kinase PINK1, whose corresponding gene is mutated in some forms of early-onset Parkinsons disease (PD). PINK1 protects cells from oxidative stress-induced cell death by suppressing release of cytochrome C from mitochondria. PD-linked PINK1 mutations impair the ability of PINK1 to phosphorylate TRAP1 and leads to impaired cell survival (4). Finally, TRAP1 alleviates α-synuclein induced toxicity and rescues the PINK1 loss-of-function phenotype (5). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Felts, S.J. et al. (2000) J Biol Chem 275, 3305-12. 2 . Hua, G. et al. (2007) J Biol Chem 282, 20553-60. 3 . Voloboueva, L.A. et al. (2008) J Cereb Blood Flow Metab 28, 1009-16. 4 . Pridgeon, J.W. et al. (2007) PLoS Biol 5, e172. 5 . Butler, E.K. et al. (2012) PLoS Genet 8, e1002488. |
Western blot analysis of extracts from various cell lines using TRAP1/HSP75 (D3D7N) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower). | |
Confocal immunofluorescent analysis of HCT 116 cells (left, high-expressing) and Hs578T cells (right, low-expressing) using TRAP1/HSP75 (D3D7N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
