| 货号 | 90944S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Monkey |
| 应用 | WB,IP,IHC |
| 目标/特异性 | HOXB13 (D7N8O) Rabbit mAb recognizes endogenous levels of total HOXB13 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) Immunohistochemistry (Paraffin) (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | HOXB13 is a member of the HOXB cluster which, along with the HOXA, HOXC, and HOXD clusters, governs embryonic patterning along the cranio-caudal axis (1,2). HOXB13 plays a key role in the development of the ventral prostate, where it is expressed highly from the embryonic stage through adulthood (3,4). Research studies have shown that both overexpression and RNA interference can inhibit the growth of prostate cancer cells. HOXB13 can function as a tumor suppressor by negatively regulating growth through repression of TCF4 and androgen receptor (AR) signaling (4,5). However, HOXB13 has also been shown to be overexpressed in more invasive prostate cancers, breast and ovarian cancers, and hepatocellular carcinomas (6-9). A common germline mutation G84E in the HOXB13 protein has recently been found to be associated with significant increased risk of prostate cancer (10). Currently, HOXB13 is being evaluated as a marker for metastatic lesions of prostate origin (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . McGinnis, W. and Krumlauf, R. (1992) Cell 68, 283-302. 2 . Zeltser, L. et al. (1996) Development 122, 2475-84. 3 . Economides, K.D. and Capecchi, M.R. (2003) Development 130, 2061-9. 4 . Jung, C. et al. (2004) Cancer Res 64, 3046-51. 5 . Jung, C. et al. (2004) Cancer Res 64, 9185-92. 6 . Jeong, T.O. et al. (2012) Mol Med Rep 5, 901-4. 7 . Shah, N. et al. (2013) Cancer Res 73, 5449-58. 8 . Miao, J. et al. (2007) Proc Natl Acad Sci U S A 104, 17093-8. 9 . Zhu, J.Y. et al. (2014) Int J Clin Exp Pathol 7, 2925-33. 10 . Ewing, C.M. et al. (2012) N Engl J Med 366, 141-9. 11 . Barresi, V. et al. (2016) APMIS 124, 188-93. 12 . Varinot, J. et al. (2016) Virchows Arch 468, 619-22. |
Immunohistochemical analysis of paraffin-embedded LNCaP cell pellet (left, positive) and DU 145 cell pellet (right, negative) using HOXB13 (D7N8O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HOXB13 (D7N8O) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HOXB13 (D7N8O) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using HOXB13 (D7N8O) Rabbit mAb. | |
Immunoprecipitation of HOXB13 from LnCAP cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HOXB13 (D7N8O) Rabbit mAb. Western blot analysis was performed using HOXB13 (D7N8O) Rabbit mAb. |
