| 货号 | 87248S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | IF |
| 目标/特异性 | NKCC2 (D5Q1H) Rabbit mAb (IF Formulated) recognizes endogenous levels of total NKCC2 protein. |
| 使用方法 | Immunofluorescence (Frozen) (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Na-K-2Cl cotransporter (NKCC2) is a sodium-potassium-chloride cotransporter. It is mainly expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henles loop (TALH) and mediates the majority of NaCl resorption and concentration of urine (1,2). NKCC2 is the target for several diuretic drugs, such as bumetanide, and is involved in the pathogenesis of hypertension (3,4). Mutations in the NKCC2-encoding gene, SLC12A1, causes Bartter’s syndrome, which is featured by impaired salt reabsorption in the TALH, hypokalemic metabolic alkalosis, and hypercalciuria (5,6). Recently, NKCC2 was reported to be expressed in the brain hypothalamo-neurohypophyseal system (HNS) and upregulated upon osmotic stress (7). |
| 存放说明 | -20C |
| 参考文献 | 1 . Igarashi, P. et al. (1996) J Biol Chem 271, 9666-74. 2 . Kaplan, M.R. et al. (1996) J Clin Invest 98, 723-30. 3 . Markadieu, N. and Delpire, E. (2014) Pflugers Arch 466, 91-105. 4 . Orlov, S.N. et al. (2015) Genes Dis 2, 186-196. 5 . Vargas-Poussou, R. et al. (1998) Am J Hum Genet 62, 1332-40. 6 . Simon, D.B. et al. (1996) Nat Genet 13, 183-8. 7 . Konopacka, A. et al. (2015) J Neurosci 35, 5144-55. |
Confocal immunofluorescent analysis of adult mouse kidney using NKCC2 (D5Q1H) Rabbit mAb (IF Formulated) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
