| 货号 | 96024S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | WB,IP,IHC |
| 目标/特异性 | CD79B (D7V2F) Rabbit mAb recognizes endogenous levels of total CD79B protein. This antibody is predicted to detect multiple isoforms of CD79B. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) IHC-Leica® Bond™ (1:100) Immunohistochemistry (Paraffin) (1:250) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The B cell antigen receptor (BCR) is composed of membrane immunoglobulin molecules non-covalently associated with the heterodimeric signaling component, CD79A and CD79B (also known as Igα and Igβ, respectively) (1,2). The presence of this receptor complex is essential for B cell development and function (3). Following antigen binding, CD79A/CD79B heterodimers are phosphorylated and initiate intracellular signaling through Src family kinases, Lyn, Blk, and Fyn, as well as Syk and Btk tyrosine kinases (4,5). The complexity of BCR signaling results in a variety of distinct cellular functions, such as proliferation, tolerance, apoptosis, and differentiation (6). BCR-antigen ligation also leads to internalization of the complex, trafficking to late endosomes, and antigen presentation in major histocompatibility molecules on the B cell surface (7,8). CD79B enhances the phosphorylation of CD79A (9). Alternatively spliced transcript variants encoding different isoforms of CD79B have been identified (10). CD79B is widely expressed on B cell malignancies and may serve as a target for therapeutic intervention (11,12). |
| 存放说明 | -20C |
| 计算分子量 | 14, 30-50 |
| 参考文献 | 1 . Mason, D.Y. et al. (1995) Blood 86, 1453-9. 2 . Storch, B. et al. (2007) Eur J Immunol 37, 252-60. 3 . Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7. 4 . Reth, M. and Wienands, J. (1997) Annu Rev Immunol 15, 453-79. 5 . Kurosaki, T. and Kurosaki, M. (1997) J Biol Chem 272, 15595-8. 6 . Dal Porto, J.M. et al. (2004) Mol Immunol 41, 599-613. 7 . Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21. 8 . van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8. 9 . Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63. 10 . Hashimoto, S. et al. (1995) Mol Immunol 32, 651-9. 11 . Dornan, D. et al. (2009) Blood 114, 2721-9. 12 . Pfeifer, M. et al. (2015) Leukemia 29, 1578-86. |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD79B (D7V2F) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD79B (D7V2F) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines and tissues using CD79B (D7V2F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of CD79B protein from Daudi cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP®Isotype Control #3900, and lane 3 is CD79B (D7V2F) Rabbit mAb. Western blot analysis was performed using CD79B (D7V2F) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using CD79B (D7V2F) Rabbit mAb performed on the Leica® BOND™ Rx. | |
Immunohistochemical analysis of paraffin-embedded Daudi cell pellets (left, positive) and Jurkat cell pellets (right, negative) using CD79B (D7V2F) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using CD79B (D7V2F) Rabbit mAb. |
