| 货号 | 17395S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Monkey |
| 应用 | WB,IP |
| 目标/特异性 | CDK8 (D6M3J) Rabbit mAb recognizes endogenous levels of total CDK8 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The mammalian Mediator Complex is a multi-subunit protein complex that couples specific transcriptional regulators to RNA polymerase II (Pol II) and the basal transcription machinery. Interactions between distinct Mediator subunits and transcription factors allow for specific gene regulation (reviewed in 1). Mediator complex interactions control various biological processes, including insulin signaling (2), NF-κB-dependent signaling (3), stem cell pluripotency and self renewal (4,5), and proliferation of colon cancer cells (6,7). CDK8/Cyclin C, along with Med12 and Med13, constitute a subcomplex within the Mediator Complex thought to act as a molecular switch, inhibiting Pol II recruitment and transcription initiation (8,9). Expression of CDK8 abrogates E2F-1-dependent inhibition of β-catenin activity in colon cancer cells (9). High levels of CDK8 coincide with high β-catenin-dependent transcription in colon cancer cells, and their proliferation can be inhibited by suppressing CDK8 expression (8). CDK8 can phosphorylate Ser727 on STAT1, which reduces natural killer (NK) cell toxicity (10,11). As such, inhibitors are being pursued as potential therapeutics to enhance NK cell activity and combat a variety of cancer types (12,13). |
| 存放说明 | -20C |
| 计算分子量 | 53 |
| 参考文献 | 1 . Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63. 2 . Wang, W. et al. (2009) Dev Cell 16, 764-71. 3 . van Essen, D. et al. (2009) PLoS Biol 7, e73. 4 . Tutter, A.V. et al. (2009) J Biol Chem 284, 3709-18. 5 . Varelas, X. et al. (2008) Nat Cell Biol 10, 837-48. 6 . Firestein, R. et al. (2008) Nature 455, 547-51. 7 . Morris, E.J. et al. (2008) Nature 455, 552-6. 8 . Knuesel, M.T. et al. (2009) Mol Cell Biol 29, 650-61. 9 . Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51. 10 . Bancerek, J. et al. (2013) Immunity 38, 250-62. 11 . Putz, E.M. et al. (2013) Cell Rep 4, 437-44. 12 . Dale, T. et al. (2015) Nat Chem Biol 11, 973-80. 13 . Rzymski, T. et al. (2017) Oncotarget , . |
Western blot analysis of extracts from HeLa, Raw 264.7, and COS-7 cell lines using CDK8 (D6M3J) Rabbit mAb. | |
Immunoprecipitation of CDK8 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is CDK8 (D6M3J) Rabbit mAb. Western blot analysis was performed using CDK8 (D6M3J) Rabbit mAb. |
