| 货号 | 17174S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB |
| 目标/特异性 | IGF-I Receptor α (D3A2W) Rabbit mAb recognizes endogenous levels of total IGF-I receptor protein. The epitope resides within the alpha subunit of the IGF-I receptor protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The type 1 insulin-like growth factor receptor (IGF1R) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell types in fetal and postnatal tissues, and which is highly similar in sequence and structure to the insulin receptor (1-4). IGF1R is synthesized as a preproprotein which is proteolytically cleaved into alpha and beta subunits. Receptor assembly involves heterodimerization of two alpha and two beta subunits to generate the heterotetrameric transmembrane receptor. The alpha subunits form the extracellular ligand binding domain; ligand binding by IGF-I or IGF-II initiates autophosphorylation of conserved intracellular residues in the beta subunit kinase domain, leading to kinase activation and subsequent activation of downstream signal transduction pathways (e.g., Akt and MAPK) (4-8). Enhanced mitogenic signaling through the IGF1R is frequently observed in cancer, making the IGF1R an important research target in translational oncology (9). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
| 参考文献 | 1 . Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93. 2 . Baserga, R. (2000) Oncogene 19, 5574-81. 3 . Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8. 4 . Massagué, J. and Czech, M.P. (1982) J Biol Chem 257, 5038-45. 5 . Ullrich, A. et al. (1986) EMBO J 5, 2503-12. 6 . Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81. 7 . Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60. 8 . Baserga, R. (1999) Exp Cell Res 253, 1-6. 9 . Iams, W.T. and Lovly, C.M. (2015) Clin Cancer Res 21, 4270-7. |
Western blot analysis of extracts from various cell lines using IGF-I Receptor α (D3A2W) Rabbit mAb. |
