| 货号 | 56439S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat |
| 应用 | WB,IP |
| 目标/特异性 | IDH2 (D8E3B) Rabbit mAb recognizes endogenous levels of total IDH2 protein. This antibody does not cross-react with IDH1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | IDH2 is one of three isocitrate dehydrogenases (IDH1-3) that catalyze the oxidative decarboxylation of isocitrate to produce CO2 and α-ketoglutarate (α-KG). These enzymes belong to two distinct subclasses that utilize either NAD or NADP+ as an electron acceptor. IDH2 is an NADP+-dependent isocitrate dehydrogenase expressed primarily in the mitochondria, where it also functions in the TCA cycle (1,2). Mutations in IDH2 or its cytoplasmic counterpart (IDH1) have been reported in glioblastoma multiforme (3), acute myeloid leukemia (4,5), and other malignancies (6). Research studies have shown that gain-of-function mutations in IDH2 can lead to the accumulation and secretion of the oncometabolite R-2-hydroxyglutarate (2HG) in cancer cells (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
| 参考文献 | 1 . Wise, D.R. et al. (2011) Proc Natl Acad Sci U S A 108, 19611-6. 2 . Filipp, F.V. et al. (2012) Pigment Cell Melanoma Res 25, 375-83. 3 . Parsons, D.W. et al. (2008) Science 321, 1807-12. 4 . Abbas, S. et al. (2010) Blood 116, 2122-6. 5 . Paschka, P. et al. (2010) J Clin Oncol 28, 3636-43. 6 . Watanabe, T. et al. (2009) Am J Pathol 174, 1149-53. 7 . Pardanani, A. et al. (2010) Leukemia 24, 1370-2. |
Western blot analysis of extracts from MCF7 and ACHN cells using IDH2 (D8E3B) Rabbit mAb. | |
Immunoprecipitation of IDH2 from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IDH2 (D8E3B) Rabbit mAb. Western blot analysis was performed using IDH2 (D8E3B) Rabbit mAb. |
