| 货号 | 53460S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB |
| 目标/特异性 | CD2 (D5L3B) Rabbit mAb recognizes endogenous levels of total CD2 protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD2 is a transmembrane glycoprotein expressed early in thymocyte development and present on most circulating T cells (1, 2). CD2 plays a role in T cell adhesion through binding to its ligand CD58 (LFA-3) (3). Stimulation of CD2 also leads to T cell activation and proliferation (2). T cells from mice deficient in both CD2 and CD28 have severe defects in T cell activation and function, while T cells deficient in either CD2 or CD28 are still capable of mounting a response, suggesting that CD2 and CD28 may have overlapping functions and may be able to compensate for each other (4). In addition, engagement of CD2 and CD58 was recently demonstrated to be the primary costimulatory signal in T cells that lack CD28 (5). CD2 expression also distinguishes a subset of plasmacytoid dendritic cells found in tumors and tonsils that express lysozyme, higher levels of IL-12 p40, and higher levels of CD80 (6). |
| 存放说明 | -20C |
| 计算分子量 | 48-65 |
| 参考文献 | 1 . Kamoun, M. et al. (1981) J Exp Med 153, 207-12. 2 . Meuer, S.C. et al. (1984) Cell 36, 897-906. 3 . Selvaraj, P. et al. Nature 326, 400-3. 4 . Green, J.M. et al. (2000) J Immunol 164, 3591-5. 5 . Leitner, J. et al. (2015) J Immunol 195, 477-87. 6 . Matsui, T. et al. (2009) J Immunol 182, 6815-23. |
Western blot analysis of extracts from various cell lines using CD2 (D5L3B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
