| 货号 | 38774S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | WB,IP |
| 目标/特异性 | CD28 (D2Z4E) Rabbit mAb recognizes endogenous levels of total CD28 protein and is predicted to react with multiple CD28 isoforms that are produced through alternative splicing. This antibody cross-reacts with 16 kDa and 100 kDa proteins of unknown origin in some mouse cells. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1, 2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6, 7). Several CD28 isoforms are produced by alternative splicing (8). |
| 存放说明 | -20C |
| 计算分子量 | 14, 40-60 |
| 参考文献 | 1 . Aruffo, A. and Seed, B. (1987) Proc Natl Acad Sci U S A 84, 8573-7. 2 . Esensten, J.H. et al. (2016) Immunity 44, 973-88. 3 . Harding, F.A. et al. (1992) Nature 356, 607-9. 4 . Azuma, M. et al. (1993) Nature 366, 76-9. 5 . Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5. 6 . Hui, E. et al. (2017) Science 355, 1428-1433. 7 . Kamphorst, A.O. et al. (2017) Science 355, 1423-1427. 8 . Magistrelli, G. et al. (1999) Biochem Biophys Res Commun 259, 34-7. |
Western blot analysis of extracts from various cell lines and primary cells using CD28 (D2Z4E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of CD28 from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) IgG XP® Isotype Control #3900, and lane 3 is CD28 (D2Z4E) Rabbit mAb. |
