| 货号 | 37359S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP,CHIP |
| 目标/特异性 | NF-κB2 p100/p52 Rabbit mAb recognizes endogenous levels of both the p100 precursor and p52 active form of NF-κB protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) Chromatin IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 120, 52 |
| 参考文献 | 1 . Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2 . Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3 . Haskill, S. et al. (1991) Cell 65, 1281-9. 4 . Thompson, J.E. et al. (1995) Cell 80, 573-82. 5 . Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6 . Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7 . Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8 . Chen, Z.J. et al. (1996) Cell 84, 853-62. 9 . Senftleben, U. et al. (2001) Science 293, 1495-9. 10 . Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11 . Xiao, G. et al. (2001) Mol Cell 7, 401-9. |
Western blot anlaysis of extracts from various cell lines using NF-κB2 p100/p52 (D7A9K) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from HDLM-2 cells and either NF-κB2 p100/p52 (D7A9K) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human IκBα Promoter Primers #5552, Human IAP2 Promoter Primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunoprecipitation of NF-κB2 p100/p52 from HDLM-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NF-κB2 p100/p52 (D7A9K) Rabbit mAb. Western blot analysis was performed using NF-κB2 p100/p52 (D7A9K) Rabbit mAb. A conformation-specific secondary antibody was used to avoid cross-reactivity with IgG. |
