| 货号 | 35509S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP |
| 目标/特异性 | LARS (D7Q4Q) Rabbit mAb recognizes endogenous levels of total LARS protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Leucyl-tRNA Synthetase (LARS) is a leucine sensor critical for the activation of mTORC1 (1). mTORC1 kinase complex is an important component in the regulation of cell growth (2,3). Its activity is modulated by energy levels, growth factors, and amino acids (4,5). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (2,3). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (2,3). LARS functions as a GTPase-activating protein (GAP) and interacts directly with RagD GTPase (1). The role of LARS in leucine sensing is not related to its tRNA charging activity (1). |
| 存放说明 | -20C |
| 计算分子量 | 134 |
| 参考文献 | 1 . Han, J.M. et al. (2012) Cell 149, 410-24. 2 . Sancak, Y. et al. (2008) Science 320, 1496-501. 3 . Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 4 . Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 5 . Wullschleger, S. et al. (2006) Cell 124, 471-84. |
Immunoprecipitation of LARS from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is LARS (D7Q4Q) Rabbit mAb. Western blot analysis was performed using LARS (D7Q4Q) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using LARS (D7Q4Q) Rabbit mAb. |
