| 货号 | 33593S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse |
| 应用 | WB,CHIP |
| 目标/特异性 | ATF-3 (D2Y5W) Rabbit mAb recognizes endogenous levels of total ATF-3 protein. |
| 使用方法 | Western Blotting (1:1000) Chromatin IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Activating transcription factor 3 (ATF-3) is a basic leucine zipper-type transcription factor belonging to the ATF/cAMP responsive element binding protein family. ATF-3 can form homodimers or heterodimers with other family members and depending on the cell types and promoter context, it can suppress or activate the transcription of its target genes (1,2). Expression of ATF-3 is induced by a variety of factors including cytokines, genotoxic agents, and physiological stress. For example, both metformin and high-density lipoprotein (HDL) can induce ATF-3 expression in macrophages (1,3-5). Research studies also indicate that ATF-3 can function as an oncogene or a tumor suppressor depending on the context (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 23, 24 |
| 参考文献 | 1 . Hai, T. et al. (1999) Gene Expr 7, 321-35. 2 . Hai, T. and Hartman, M.G. (2001) Gene 273, 1-11. 3 . Hai, T. et al. (2010) Gene Expr 15, 1-11. 4 . De Nardo, D. et al. (2014) Nat Immunol 15, 152-60. 5 . Kim, J. et al. (2014) J Biol Chem 289, 23246-55. 6 . Yin, X. et al. (2008) Oncogene 27, 2118-27. 7 . Wang, Z. et al. (2014) Oncogene Advanced online publication, doi:10.1038/onc.2014.426. |
Western blot analysis of extracts from 293T and SR cells using ATF-3 (D2Y5W) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either ATF-3 (D2Y5W) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ATF-3 Exon 1 Primers #66427, human RUNX2 intron 5 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
