| 货号 | 78128S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP |
| 目标/特异性 | MCRIP1 (D2Y8V) Rabbit mAb recognizes endogenous levels of total MCRIP1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mapk-regulated corepressor-interacting protein 1 (MCRIP1) is a widely expressed protein substrate of ERK. MCRIP1 functions in regulating transcriptional repression of E-cadherin by the transcriptional corepressor CtBP. Unphosphorylated MCRIP1 binds to CtBP, preventing its transcriptional regulatory activity. When MCRIP1 is phosphorylated by ERK, CtIP is free to interact with the transcriptional repressor, ZEB1. The CtIP/ZEB1 complex then silences E-cadherin expression, promoting epithelial-mesenchymal transition (EMT), a process in tumor progression that leads to enhanced migration and invasion (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 11 |
| 参考文献 | 1 . Ichikawa, K. et al. (2015) Mol Cell 58, 35-46. 2 . Yeung, K.T. and Yang, J. (2017) Mol Oncol 11, 28-39. |
Western blot analysis of extracts from various cell lines using MCRIP1 (D2Y8V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of MCRIP1 protein from NCI-H1299 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is MCRIP1 (D2Y8V) Rabbit mAb. Western blot analysis was performed using |
