| 货号 | 78105S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | CHIP |
| 目标/特异性 | BAP1 (D1W9B) Rabbit mAb recognizes endogenous levels of total BAP1 protein. |
| 使用方法 | Chromatin IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | BRCA1-Associated Protein 1 (BAP1) was originally identified as a BRCA1 associated, nuclear localized ubiquitin hydrolase that suppresses cell growth (1). The protein belongs to the UCH family of deubiquitinases, with a UCH domain in its amino-terminal segment and a BRCA1 interaction domain as well as a nuclear localization signal in its carboxy-terminal segment (1). Frequent gene locus rearrangement, deletion, and null mutation of BAP1 have been found in lung and breast cancers (1,2). In vivo mutation analysis of cancer cell line survival and animal tumorigenesis indicates that both the deubiquitinase activity and the nuclear localization signal are required for BAP1 function as a tumor suppressor (3). BAP1 does not have direct deubiquitination activity towards the autoubiquitinated BRCA1/BARD1 E3 complex (4), but its interaction with BARD1 inhibits BRCA1/BARD1 E3 activity by interfering with the complex dimerization process (5). In addition to its interaction with BRCA1/BARD1, BAP1 has also been shown to interact with and deubiquitinate HCF-1, thereby controlling its stability (6). |
| 存放说明 | -20C |
| 参考文献 | 1 . Jensen, D.E. et al. (1998) Oncogene 16, 1097-112. 2 . Buchhagen, D.L. et al. (1994) Int J Cancer 57, 473-9. 3 . Ventii, K.H. et al. (2008) Cancer Res 68, 6953-62. 4 . Mallery, D.L. et al. (2002) EMBO J 21, 6755-62. 5 . Nishikawa, H. et al. (2009) Cancer Res 69, 111-9. 6 . Misaghi, S. et al. (2009) Mol Cell Biol 29, 2181-92. |
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either BAP1 (D1W9B) Rabbit mAb (ChIP Formulated) or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ID1 5UTR Primers #89001, human ID2 promoter primers, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
