| 货号 | 77694S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | WB,IP |
| 目标/特异性 | CHD8 (D5E1W) Rabbit mAb recognizes endogenous levels of total CHD8 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains, and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10). |
| 存放说明 | -20C |
| 计算分子量 | 290 |
| 参考文献 | 1 . Hargreaves, D.C. and Crabtree, G.R. (2011) Cell Res 21, 396-420. 2 . Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40. 3 . Delmas, V. et al. (1993) Proc Natl Acad Sci U S A 90, 2414-8. 4 . Woodage, T. et al. (1997) Proc Natl Acad Sci U S A 94, 11472-7. 5 . Yates, J.A. et al. (2010) FEBS Lett 584, 689-93. 6 . Thompson, B.A. et al. (2008) Mol Cell Biol 28, 3894-904. 7 . Nishiyama, M. et al. (2009) Nat Cell Biol 11, 172-82. 8 . Caldon, C.E. et al. (2009) Mol Cell Biol 29, 4623-39. 9 . Menon, T. et al. (2010) Mol Endocrinol 24, 1165-74. 10 . Ishihara, K. et al. (2006) Mol Cell 23, 733-42. |
Western blot analysis of extracts from various cell lines using CHD8 (D5E1W) Rabbit mAb. | |
Immunoprecipitation of CHD8 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CHD8 (D5E1W) Rabbit mAb. Western blot analysis was performed using CHD8 (D5E1W) Rabbit mAb. |
