5-Lipoxygenase (C49G1) Rabbit mAb【科瑞奥博】

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5-Lipoxygenase (C49G1) Rabbit mAb

货号： 3289S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	3289S
反应种属	Human
来源宿主	Rabbit
应用	W/IP/IHC-P
目标/特异性	5-Lipoxygenase (C49G1) Rabbit mAb detects endogenous levels of total 5-lipoxygenase protein. Expression of 5-Lipoxygenasae is very low in most tissues and cell lines, excepting whole blood, bone marrow, lung and macrophage cell lines.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:50)

性能
供应商	CST
背景	5-Lipoxygenase (5-LO, ALOX5) is an important catalytic enzyme responsible for the biosynthesis of leukotriene LTA4 from arachidonic acid (1,2). Leukotriene synthesis also requires 5-lipoxygenase-activating protein (FLAP, ALOX5AP), a nuclear membrane-bound protein that binds arachidonic acid and is thought to activate 5-LO. A number of related leukotrienes (i.e. B4, C4, D4) are derived from LTA4 and together these lipid mediators function in immune reaction regulation. 5-LO is primarily expressed in polymorphonuclear leukocytes, peripheral blood monocytes, macrophages, and mast cells (1,3). Overexpression of 5-LO protein is seen in certain cancer cells and is associated with poor diagnosis (1,4). Depending upon the cell type, 5-LO is localized to either the cytosol or the nucleus of quiescent cells (5). Following stimulation, 5-LO translocates to the nucleus and associates with FLAP to catalyze LTA4 synthesis (2,3). Phosphorylation of specific residues can regulate 5-LO enzymatic activity. Phosphorylation of 5-LO at Ser523 by PKA family kinases inhibits oxygenase activity (6,7) while MAPKAP2 and ERK family kinase phosphorylation at Ser271 and Ser663 stimulates 5-LO enzymatic activity in vivo (8,9).
存放说明	-20C
计算分子量	78

参考图片

Western blot analysis of extracts from COS cells transfected with 5-LO using 5-Lipoxygenase (49G1) Rabbit mAb.Western免疫印迹。用5-Lipoxygenase (49G1) Rabbit mAb研究转染了5-LO COS细胞的细胞提取液。

Western blot analysis of extracts from human RL cells using 5-Lipoxygenase (49G1) Rabbit mAb.Western免疫印迹。用5-Lipoxygenase (49G1) Rabbit mAb研究人的RL细胞的细胞提取液。

Immunohistochemical analysis of paraffin-embedded non-Hodgkins lymphoma, using 5-Lipoxygenase (C49G1) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).免疫组织化学染色分析石蜡包埋人非霍奇金淋巴瘤组织。在对照多肽 (左图)和抗原特异性多肽 (右图)存在下。所用抗体为5-Lipoxygenase (C49G1) Rabbit mAb。