| 货号 | 13294S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | ACAT2 (E1L8V) Rabbit mAb recognizes endogenous levels of total ACAT2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Acetyl-CoA acetyltransferase 2 (ACAT2), also known as cytosolic acetoacetyl-CoA thiolase, plays a role in regulating lipid metabolism (1). It catalyzes the synthesis of acetoacetyl-CoA from two acetyl-CoA molecules, which is later converted into steroids (2). |
| 存放说明 | -20C |
| 计算分子量 | 41 |
Western blot analysis of extracts from various cell lines using ACAT2 (E1L8V) Rabbit mAb. 使用ACAT2 (E1L8V) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human ACAT2 (hACAT2-DDK; +), using ACAT2 (E1L8V) Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). 模拟转染(-)或转染了表达DDK标记的full-length human ACAT2 质粒(hACAT2-DDK; +)的293细胞提取物，使用ACAT2 (E1L8V) Rabbit mAb兔单抗 (上), DYKDDDDK Tag Antibody #2368 (中), 或 GAPDH (D16H11) XP® Rabbit mAb 兔单抗#5174 (下)进行western blot分析。 | |
Immunoprecipitation of ACAT2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ACAT2 (E1L8V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ACAT2 (E1L8V) Rabbit mAb. 使用Rabbit (DA1E) mAb兔单抗 IgG XP® Isotype Control #3900 (lane 2) 或ACAT2 (E1L8V) Rabbit mAb兔单抗 (lane 3)对Hep G2细胞的ACAT2进行免疫沉淀实验。Lane1是10%上样量。使用ACAT2 (E1L8V) Rabbit mAb兔单抗进行western blot分析。 |
