| 货号 | 12019S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | ADRM1 (D9Z1U) Rabbit mAb recognizes endogenous levels of total ADRM1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Currently, there are five ubiquitin receptors associated with the proteasome: two proteasome subunits, Rpn10/S5a/PSMD4 and Rpn13/ADRM1 (Adhesion-regulating molecule 1), and three families of shuttling factors, Rad23, Dsk2, and Ddi1. ADRM1 is a ubiquitin receptor of the proteasome (1,2) that binds ubiquitin via a pleckstrin homology domain known as the pleckstrin-like receptor for ubiquitin (Pru) domain. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37 (3-5), perhaps serving as a mechanism to accelerate ubiquitin chain disassembly. A murine Adrm1 knockout results in defective gametogenesis, thus highlighting a physiologic role for endogenous ADRM1 in mammalian development (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from various cell lines using ADRM1 (D9Z1U) Rabbit mAb. Western blot检测多种细胞提取物，使用的抗体是ADRM1 (D9Z1U) Rabbit mAb。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ADRM1 (hADRM1-Myc/DDK; +), using ADRM1 (D9Z1U) Rabbit mAb. Western blot方法检测mock 转染的 (-) 或转染表达Myc/DDK标记的全长人ADRM1(hADRM1-Myc/DDK; +)的293T细胞，所用的抗体是ADRM1 (D9Z1U) Rabbit mAb。 | |
Immunoprecipitation of ADRM1 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ADRM1 (D9Z1U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot detection was performed using ADRM1 (D9Z1U) Rabbit mAb. 免疫沉淀检测293T细胞提取物，所用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control#3900 (lane 2)或ADRM1 (D9Z1U) Rabbit mAb (lane 3)。Lane 1中样品浓度为10%的Input。Western blot 检测所用的抗体是ADRM1 (D9Z1U) Rabbit mAb。 |
