| 货号 | 13531S |
| 反应种属 | Human/Mouse/Rat/Monkey/Dog |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | <p>In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium (reviewed in 1). Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet (reviewed in 2). The actin filament-binding protein, Afadin, binds to nectin forming a connection to the actin cytoskeleton (3). AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these junctions are then involved in the formation and maintenance of TJs (4,5). Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin, as well as the carboxyl-terminal F-actin binding region (6). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (7). Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (8,9).</p>在多细胞生物中，细胞间连接在组织完整性和未出细胞极性过程中发挥了关键作用。紧密连接(TJs)形成一个跨越上皮和内皮细胞的持续性屏障阻挡液体(reviewed in 1)。黏附连接（AJs）是一个动态结构形成细胞-细胞间连接将细胞和一个持续的薄片连接（reviewed in 2）。肌动蛋白丝结合蛋白，Afadin，和nectin结合后形成一个连接与肌动蛋白丝细胞骨架连接(3)。当nectin募集钙粘蛋白到细胞-细胞黏附位置时会形成AJs，这些连接随后会涉及到TJs的形成和维持(4,5). Afadin有两种剪切异构体：l-afadin广泛表达以及s-afadin主要表的在神经组织中。s-afadin是I-afadin的的一个缩短形式，缺少了一个三脯氨酸富集区域和羧基端的F-actin结合区域（6）。人s-afadin与AF-6相同，这个ALL-1融合伴侣涉及到急性白血病的发生（7）。最近的工作证明当afadin位于运动细胞的前端时，参与控制细胞运动的方向（8,9）。 |
| 存放说明 | -20C |
| 计算分子量 | 205 |
Western blot analysis of extracts from various cell lines using Afadin (D1Y3Z) Rabbit mAb.使用Afadin (D1Y3Z) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of afadin from A-431 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Afadin (D1Y3Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Afadin (D1Y3Z) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 Afadin (D1Y3Z) Rabbit mAb (lane 3)对A-431细胞提取物中的afadin进行免疫沉淀实验。Land1是10%上样量。使用Afadin (D1Y3Z) Rabbit mAb进行western blot分析。 |
