| 货号 | 12684S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | AFAP1L2/XB130 (D1B5) Rabbit mAb recognizes endogenous levels of total AFAP1L2/XB130 protein. In some cell extracts the antibody recognizes an 80 kDa background protein of unknown origin. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The actin-filament associated protein (AFAP) family consists of AFAP1, AFAP1L1, and AFAP1L2/XB130, a group of structurally similar proteins that play distinct roles in the regulation of cytoskeletal dynamics and signal transduction. Actin filament-associated protein 1-like 2 (AFAP1L2, XB130) is an adaptor protein that regulates signaling downstream of multiple kinases, including Src, Akt, and the thyroid specific kinase RET/PTC (1-3). Through these pathways, AFAP1L2/XB130 mediates transcriptional regulation, cell proliferation, motility, and microRNA expression (4,5). Research has shown that AFAP1L2/XB130 is involved in the proliferation and survival of thyroid tumor cells (6), and may have value in gastric cancer prognosis (7). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Western Blot analysis of extracts from various cell types using AFAP1L2/XB130 (D1B5) Rabbit mAb.使用AFAP1L2/XB130 (D1B5) Rabbit mAb对多种细胞系提取物进行western blot分析。 | |
Immunoprecipitation of AFAP1L2/XB130 from A-431 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AFAP1L2/XB130 (D1B5) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using AFAP1L2/XB130 (D1B5) Rabbit mAb.使用 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或AFAP1L2/XB130 (D1B5) Rabbit mAb（lane3）对A-431细胞提取物中的AFAP1L2/XB130进行免疫沉淀实验。Lane1是10%上样量。AFAP1L2/XB130 (D1B5) Rabbit mAb也用于western blot分析。 |
