| 货号 | 4975S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | AID (L7E7) Mouse mAb detects endogenous levels of AID protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation (1). The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function (2). AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma) (3). |
| 存放说明 | -20C |
| 计算分子量 | 24 |
Western blot analysis of extracts from P3HR-1 and Ramos cells using AID (L7E7) Mouse mAb.Western免疫印迹。用 AID (L7E7) Mouse mAb研究P3HR-1 和 Ramos 细胞的细胞提取液。 | |
Western blot analysis of immunoprecipitates from HeLa cell extracts overexpressing myc-tagged AID. Expression of myc-AID was confirmed (lane 1). Immunoprecipitations using cell lysates with or without expression of myc-AID were performed, using AID (L7E7) Mouse mAb #4975, Myc-Tag Antibody #2272, mouse IgG or rabbit IgG. Immunoblot was performed using Myc-Tag Antibody #2272.Western免疫印迹分析过表达myc-AID HeLa细胞提取液的免疫沉淀，所用抗体为Myc-Tag Antibody #2272。证实myc-AID的表达(泳道1)。 用AID (L7E7) Mouse mAb #4975, Myc-Tag Antibody #2272, mouse IgG 或rabbit IgG免疫沉淀未表达和表达了myc-AID的HeLa细胞的细胞提取液。 |
