| 货号 | 4685S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | Akt (pan) (11E7) Rabbit mAb detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Flow cytometric analysis of untreated Jurkat cells, using Akt (pan) (11E7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 应用Akt (pan) (11E7) Rabbit mAb (蓝色) 流式细胞术分析未处理的Jurkat 细胞，并和非特异阴性对照抗体（红色）比较。 | |
Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins, and extracts from HeLa, C2C12, C6 and COS cells, using Akt (pan) (11E7) Rabbit mAb. Western blot 分析重组的Akt1, Akt2和 Akt3蛋白，以及HeLa, C2C12, C6 和COS细胞的提取物，使用的抗体为Akt (pan) (11E7) Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or insulin-treated (right), using Akt (pan) (11E7) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 免疫荧光共聚焦分析HeLa 细胞，左图为血清饥饿型，右图为胰岛素处理组，使用的抗体为Akt (pan) (11E7) Rabbit mAb 兔单抗（绿色）。 Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。 | |
Immunohistochemical analysis using Akt (pan) (11E7) Rabbit mAb on SignalSlide(TM) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)). Note the lack of phosphorylated Akt-associated stain at the membrane of the LY294002 treated cells. 免疫组化分析石蜡包埋的人LNCaP细胞，左图为未处理组，右图为LY294002处理组，使用的抗体为Akt (pan) (11E7) Rabbit mAb兔单抗。注意:在LY294002处理的细胞膜上没有磷酸化Akt相关的染色。 | |
Immunohistochemical analysis of paraffin-embedded HeLa cells untreated (left) or transfected with Akt 1/2/3 Kinases ShortCut® siRNA Mix (New England BioLabs #N2005) (right), using Akt (pan) (11E7) Rabbit mAb (top) or Cleaved Caspase-3 (Asp175) #9661 (bottom). Note the induction of cleaved caspase-3 in Akt deficient cells. | |
免疫组化分析石蜡包埋的Hela细胞，作图为未处理组，右图为Akt 1/2/3 Kinases ShortCut® siRNA Mix (New England BioLabs #N2005) 处理组，所用抗体为Akt (pan) (11E7) Rabbit mAb兔单抗 (上) or Cleaved Caspase-3 (Asp175) #9661 (下)。注意Akt缺失细胞中caspase-3剪切体的诱导 。 |
