| 货号 | 2920S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | Akt (pan) (40D4) Mouse mAb detects endogenous levels of total Akt protein. This antibody does not cross-react with other related proteins. |
| 使用方法 | WB(1:2000) IP (1:50) IHC-P (1:250) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Flow cytometric analysis of untreated Jurkat cells using Akt (pan) (40D4) Mouse mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞技术分析未处理的Jurkat细胞；使用的抗体是 Akt (pan) (40D4) Mouse mAb鼠单抗（蓝色），非特异性的阴性对照抗体（红色）。 | |
Western blot analysis of extracts from NIH/3T3, C6 and COS cells using Akt (pan) (40D4) Mouse mAb. Western blot检测分析NIH/3T3, C6 和 COS细胞提取物。使用抗体是Akt (pan) (40D4) Mouse mAb鼠单抗。 | |
Confocal immunofluorescent analysis of C2C12 cells, either LY294002-treated (left) or insulin-treated (right), using pan-Akt (40D4) MmAb (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 激光共聚焦荧光法检测C2C12细胞，左图为LY294002处理的，右图为insulin处理的，使用的抗体是pan-Akt (40D4) MmAb（绿色）。微丝用DY-554 phalloidin标记（红色）。蓝色伪彩是 DRAQ5™ (fluorescent DNA dye)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Akt (pan) (40D4) Mouse mAb in the presence of control peptide (left) or Akt (pan) Blocking Peptide #1085 (right). | |
免疫组织化学方法检测石蜡包埋的人乳腺癌组织，左图是对照肽段处理的，右图是Akt (pan) Blocking Peptide #1085处理的；使用的抗体是Akt (pan) (40D4) Mouse mAb鼠单抗。 |
