| 货号 | 13185S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F |
| 目标/特异性 | AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb recognizes endogenous levels of total AMPA Receptor (GluR 1) protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-F (1:200) |
| 供应商 | CST |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Confocal immunofluorescent analysis of mouse hippocampus using AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析小鼠海马组织，使用的抗体是AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb 兔单抗 (绿色)。蓝色伪彩= DRAQ5® #4084 (荧光 DNA 染料)。 | |
Immunoprecipitation of AMPA Receptor (GluR 1) from mouse brain extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb兔单抗 (lane 3)对小鼠大脑组织提取物中的AMPA Receptor (GluR 1)进行免疫沉淀。Lane1是10%上样量。使用AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb 兔单抗进行western blot分析。 | |
Western blot analysis of extracts from mouse brain, rat brain, and rat prefrontal cortex tissues using AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb. 使用AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb. Western blot分析小鼠脑组织，大鼠脑组织和大鼠前额叶皮层组织提取物，使用的抗体是AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb 兔单抗。 |
