| 货号 | 14476S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Anoctamin 1, also called TMEM16A for Transmembrane Member 16A, is a plasma membrane calcium-activated chloride channel (1). It is essential for chloride secretion from epithelial tissues (2), and has been shown to be a specific marker for cells of Cajal (3), the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction (4). More recently, research studies have identified Anoctamin 1 as a heat sensor in nociceptive neurons (5). Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception (5). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
Confocal immunofluorescent analysis of COLO 205 (positive, left) and HeLa (negative, right) cells using Anoctamin 1 (D1M9Q) Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various tissues and cell lines using Anoctamin 1 (D1M9Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of anoctamin 1 from HT-29 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Anoctamin 1 (D1M9Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Anoctamin 1 (D1M9Q) Rabbit mAb. |
