| 货号 | 13329S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | APC1 (D1E9D) Rabbit mAb recognizes endogenous levels of total APC1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex found in the nucleus of interphase cells. This complex diffuses throughout the cytoplasm and associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity is also strictly dependent upon one of several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1, which contain a carboxy-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8). |
| 存放说明 | -20C |
| 计算分子量 | 216 |
Western blot analysis of extracts from various cell lines using APC1 (D1E9D) Rabbit mAb.Western blot检测多种细胞提取物，使用的抗体是APC1 (D1E9D) Rabbit mAb. | |
Immunoprecipitation of APC1 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or APC1 (D1E9D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using APC1 (D1E9D) Rabbit mAb.对293T细胞提取物中的APC1进行免疫共沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或APC1 (D1E9D) Rabbit mAb (泳道3)。泳道1为10%的上样。Western blot分析使用抗体是APC1 (D1E9D) Rabbit mAb。 | |
Western blot analysis of extracts from 293T cells, transfected with 100nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® APC1 siRNA I #13441 (+), or SignalSilence® siRNA II #13506 (+), using APC1 (D1E9D) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The APC1 (D1E9D) Rabbit mAb confirms silencing of APC1, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.Western blot检测293T细胞提取物，转染有100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)、SignalSilence® APC1 siRNA I #13441 (+), 或 SignalSilence® APC1 siRNA II #13506 (+)，使用的抗体是APC1 (D1E9D) Rabbit mAb (上图) 或 GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。APC1 (D1E9D) Rabbit mAb证实了APC1沉默表达，采用GAPDH (D16H11) XP® Rabbit mAb做对照。 |
