| 货号 | 14090S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Cell proliferation in all eukaryotic cells relies, in part, upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C). The main function of the APC/C is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex that associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity also relies upon several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1, which contain a carboxy-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8). |
| 存放说明 | -20C |
| 计算分子量 | 10 |
Western blot analysis of extracts from various cell lines using APC11 (D1E7Q) Rabbit mAb. | |
Immunoprecipitation of APC11 from 293T cell extracts using either Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or APC11 (D1E7Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using APC11 (D1E7Q) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® APC11 siRNA I #14100 (+), using APC11 (D1E7Q) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The APC11 (D1E7Q) Rabbit mAb confirms silencing of APC11, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control. |
