| 货号 | 12530S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | APC3 (D3I1V) Rabbit mAb recognizes endogenous levels of total APC3 protein. This antibody does not cross-react with either APC8/CDC23 or APC6/CDC16. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex found in the nucleus of interphase cells. This complex diffuses throughout the cytoplasm and associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity is also strictly dependent upon one of several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1/FZR1, which contain a C-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8). |
| 存放说明 | -20C |
| 计算分子量 | 97 |
Western blot analysis of extracts from various cell lines using APC3 (D3I1V) Rabbit mAb.Western blot检测多种细胞提取物。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human APC3 (hAPC3-Myc/DDK; +), using APC3 (D3I1V) Rabbit mAb. Western blot检测mock transfected (-)或转染表达Myc/DDK-tagged全长人APC3 (hAPC3-Myc/DDK; +)。 | |
Immunoprecipitation of APC3 from MCF7 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or APC3 (D3I1V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using APC3 (D3I1V) Rabbit mAb.Immunoprecipitation检测MCF7细胞提取物，采用Rabbit (DA1E) mAb IgG XP®同型对照#3900 (lane 2) 或APC3 (D3I1V) Rabbit mAb (lane 3)。Lane 1中样品浓度为10%。Western blot检测采用APC3 (D3I1V) Rabbit mAb。 |
