| 货号 | 9499S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex found in the nucleus of interphase cells. This complex diffuses throughout the cytoplasm and associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity is also strictly dependent upon one of several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1, which contain a C-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-Boxes (7) and KEN-boxes (8).所有真核细胞的增殖严格取决于细胞分裂后期促进复合体的泛素连接酶（E3）的活性，该复合体的主要功能是触发细胞周期从中期向后期过渡。 APC/C是在有丝分裂中期细胞核中发现的一种1.5 MDa的蛋白质。该复合体分散在整个细胞质并和有丝分裂过程中的纺锤体部分相联系。APC/C通过促进底物蛋白上多聚泛肽链的装配来执行其各种功能，从而靶向这些蛋白质并通过26S蛋白酶体降解（1,2）。在人类中，12个不同的APC/ C亚基已经鉴定。像所有的E3酶一样，APC/C能够利用素E1酶激活的泛素蛋白残基，然后转移到E2酶。事实上，APC/C已被证明能够瞬时与UBCH5和UBCH10 E2酶相互作用，部分通过含有APC11亚基的RING-指结构域（3-5）。除E2酶之外，APC/C的活性也严格取决于细胞周期的特定阶段联合APC/ C的几个辅助因子中的一个。这些蛋白中研究比较到位的是Cdc20 和Cdh1，其中含有C-末端的WD40结构域和参与APC/C底物的识别，通过与这些底物中的特定识别元件相互作用(6)，称为D-盒（7），以及KEN盒（8）。 |
| 存放说明 | -20C |
| 计算分子量 | 72 |
Western blot analysis of extracts from various cell lines using APC6 (D8D8) Rabbit mAb.Western blot方法检测不同细胞提取物，使用的抗体为 APC6 (D8D8) Rabbit mAb。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human APC6 (hAPC6-Myc/DDK, +), using APC6 (D8D8) Rabbit mAb.Western blot方法检测293细胞提取物，分为空对照转染组(-)合编码人全称APC6蛋白的带有Myc/DDK-标签的cDNA 转染组(hAPC6-Myc/DDK, +)，使用的抗体为APC6 (D8D8) Rabbit mAb。 |
