| 货号 | 8941S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | ApoE4 (4E4) Mouse mAb recognizes endogenous levels of total ApoE4 protein. This antibody does not cross-react with ApoE2 or ApoE3. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2). Human ApoE has three isoforms: ApoE2, ApoE3, and ApoE4. These three isoforms differ in the combination of cysteine and arginine residues located at positions 130 and 176. The ApoE4 isoform contains arginine at both locations. Research studies have linked ApoE4 function to neuronal plasticity, synaptogenesis, and neurodegenerative diseases (3). ApoE4 is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Investigators have established the ApoE4 allele as a genetic risk factor for Alzheimer’s disease (AD), accounting for 50-60% of the genetic variation in the disease (4). Research studies indicate that patients expressing ApoE4 have a reduced capacity for synaptic plasticity, an earlier age of onset of AD, and an increase in amyloid-beta (Aβ) deposition. The increase in Aβ suggests a role for ApoE4 in the impairment of amyloid clearance (5). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
Western blot analysis of extracts from HeLa cells, transfected with a construct expressing full-length human ApoE2 (hApoE2; +), ApoE3 (hApoE3; +), or ApoE4 (hApoE4; +), using ApoE4 (4E4) Mouse mAb (upper) or a pan ApoE antibody (lower). Western blot方法检测HeLa细胞提取物：全长人ApoE2 (hApoE2; +)，ApoE3 (hApoE3; +)或ApoE4 (hApoE4; +)质粒转染；使用的抗体是ApoE4 (4E4) Mouse mAb鼠单抗（上图）或一个广谱型ApoE抗体（下图）。 | |
Western blot analysis of human serum using ApoE4 (4E4) Mouse mAb. Western blot 检测人血清样品，使用的抗体是ApoE4 (4E4) Mouse mAb鼠单抗。 |
