| 货号 | 13357S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | ARD1A (E1J2B) Rabbit mAb recognizes endogenous levels of total ARD1A protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Protein acetylation is a common modification that occurs both at lysine residues within proteins (ε-amino acetylation) and multiple amino acid residues at the amino terminus of proteins (α-amino acetylation). The N-α-acetyltransferase ARD1 homolog A protein (ARD1A, also known as NAA10) and the highly homologous N-α-acetyltransferase ARD1 homolog B protein (ARD1B, also known as ARD2 or NAA11) are mutually exclusive catalytic subunits of the amino-terminal acetyltransferase complex (NatA) (1-3). This complex, which consists of either ARD1A or ARD1B and the N-α-acetyltransferase 15 (NAA15) auxiliary protein, localizes to ribosomes where it functions to acetylate Ser-, Ala-, Gly-, Thr-, Cys-, Pro-, and Val- amino termini after initiator methionine cleavage during protein translation (1-5). Like ε-amino acetylation, amino-terminal α-amino acetylation functions to regulate protein stability, activity, cellular localization, and protein-protein interactions (4,5). Defects in ARD1A have been shown to cause amino-terminal acetyltransferase deficiency (NATD), which results in severe delays and defects in postnatal growth (6). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
Western blot analysis of extracts from various cell lines using ARD1A (E1J2B) Rabbit mAb.Western blot方法检测多种细胞系的提取物，使用的抗体为ARD1A (E1J2B) Rabbit mAb。 | |
Immunoprecipitation of ARD1A from 293 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ARD1A (E1J2B) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ARD1A (E1J2B) Rabbit mAb.从293细胞提取物中免疫沉淀ARD1A，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 ARD1A (E1J2B) Rabbit mAb (lane 3)。Lane 1为10% input。 使用ARD1A (E1J2B) Rabbit mAb进行Western blot检测。 |
