| 货号 | 5054S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Argonaute 3 (D15D2) Rabbit mAb recognizes endogenous levels of total Argonaute 3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1). |
| 存放说明 | -20C |
| 计算分子量 | 97 |
Western blot analysis of extracts from PANC-1, HeLa, and C2C12 cells using Argonaute 3 (D15D2) Rabbit mAb. blot方法检测PANC-1, HeLa, 及 C2C12 细胞的提取物，使用的抗体为Argonaute 3 (D15D2) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human Argonaute 3 (hArgonaute 3-DDK; +), using Argonaute 3 (D15D2) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot方法检测293细胞提取物，细胞分别使用空白对宅（-）或构建的DDK标签的全长人ELAVL1/HuR Argonaute 3 (hArgonaute 3-DDK; +)转染，使用的抗体为Argonaute 3 (D15D2) Rabbit mAb兔单抗 (上图), DYKDDDDK Tag (9A3) Mouse mAb鼠单抗 #8146 (中),或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下图)。 |
