| 货号 | 12354S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | ARID1A/BAF250A (D2A8U) Rabbit mAb recognizes endogenous levels of total ARID1A/BAF250A protein. This antibody also cross-reacts with proteins of unknown origin at 65 kDa. |
| 使用方法 | WB(1:1000) IHC-P (1:500) |
| 供应商 | CST |
| 背景 | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). |
| 存放说明 | -20C |
| 计算分子量 | 250 |
Western blot analysis of extracts from various cell lines using ARID1A/BAF250A (D2A8U) Rabbit mAb. Western blot检测各种细胞系提取物,使用ARID1A/BAF250A(D2A8U) Rabbit mAb鼠单抗。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, COS-7 (left) or T-47D (right), using ARID1A/BAF250A (D2A8U) Rabbit mAb. 石蜡包埋细胞的免疫组化，cos-7(左)或T-47D(右)，使用抗体为ARID1A/BAF250A(D2A8U) Rabbit mAb兔单抗. | |
Immunohistochemical analysis of paraffin-embedded human lung adenosquamous carcinoma using ARID1A/BAF250A (D2A8U) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 人肺腺癌的免疫组化石蜡切片，使用抗体为ARID1A/BAF250A(D2A8U) Rabbit mAb兔单抗，在对照肽段中有表达（图片左），右图为抗原特异肽段。 | |
Western blot analysis of T-47D and Jurkat cell extracts using ARID1A/BAF250A (D2A8U) Rabbit mAb (upper) or ß-Actin (D6A8) Rabbit mAb #8457 (lower). Additional ARID1A/BAF250A degradation products may be detected in some cell extracts between 135kDa-250kDa, which are absent in the ARID1A/BAF250A negative T-47D cell line. Western blot方法检测T-47D和Jurkat细胞系的提取物，使用的抗体为ARID1A/BAF250A(D2A8U) rabbit mAb兔单抗(上面)或者β-actin(D6A8) rabbit mAb#8457兔单抗（下面）。一些ARID1A/BAF250A降解的产物可能在一些细胞提取物中检测到，分子量位于135kDa到250kDa之间，但是在T-47D细胞系中检测不到。 |
