| 货号 | 2990S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | ASF1A (C6E10) Rabbit mAb detects endogenous levels of total ASF1A protein. The antibody does not cross-react with ASF1B protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | ASF1 was first identified in S. cerevisiae based on its ability to de-repress transcriptional silencing when overexpressed (1). While only one gene exists in yeast and Drosophila, mammalian cells contain the two highly homologous ASF1A and ASF1B genes (2). ASF1A and ASF1B function as histone chaperones, delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA (2-5). Both ASF1A and ASF1B bind to CAF-1, but only ASF1A binds to HIRA (5). In addition to playing a role in DNA replication and gene silencing, ASF1 functions in DNA damage repair, genome stability and cellular senescence. Deletion of ASF1 in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint (6-8). Depletion of both ASF1A and ASF1B in mammalian cells results in the accumulation of cells in S phase, increased phosphorylation of H2A.X, centrosome amplification and apoptosis (9,10). ASF1A is required for the formation of senescence-associated heterochromatin foci (SAHF), with overexpression of ASF1A inducing senescence in primary cells (4). Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress (11,12). The function of ASF1 phosphorylation is not yet understood. |
| 存放说明 | -20C |
| 计算分子量 | 20 |
Immunohistochemical analysis of paraffin-embedded human melanoma using ASF1A (C6E10) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right). | |
使用ASF1A (C6E10) Rabbit mAb兔单抗，其分别与control peptide (左图)或抗原特异性肽段 (右图)孵育，免疫组化分析人源黑素瘤组织石蜡切片。 | |
