| 货号 | 13492S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Atg101 (E1Z4W) Rabbit mAb recognizes endogenous levels of total Atg101 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 25 |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Atg101 protein (hAtg101-Myc/DDK; +), using Atg101 (E1Z4W) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). Western blot分析293细胞的细胞提取物，未转染（-）对照或转染Myc/DDK标签的全长人源的Atg101蛋白(hAtg101-Myc/DDK; +) 载体，使用的抗体是Atg101 (E1Z4W) Rabbit mAb 兔单抗（上图）和Myc-Tag (71D10) Rabbit mAb 兔单抗#2278（下图）。 | |
Western blot analysis of extracts from various cell lines using Atg101 (E1Z4W) Rabbit mAb. Western blot 分析多种细胞系的细胞提取物，使用的抗体是Atg101 (E1Z4W) Rabbit mAb 兔单抗。 |
