| 货号 | 13468S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Atg13 (E1Y9V) Rabbit mAb recognizes endogenous levels of total Atg13 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. |
| 存放说明 | -20C |
| 计算分子量 | 72 |
Confocal immunofluorescent analysis of PANC-1 cells, untreated (left) or nutrient-starved with Earles balanced salt solution (3 hr; right), using Atg13 (E1Y9V) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦试验分析PANC-1细胞，未处理（左图）或Earles平衡盐溶液营养缺乏处理（3小时；下图），使用抗体是Atg13 (E1Y9V) Rabbit mAb 兔单抗（绿色）。蓝色伪彩=DRAQ5® #4084（荧光DNA染料）。 | |
Western blot analysis of extracts from various cell lines using Atg13 (E1Y9V) Rabbit mAb. Western blot 分析多种细胞系的细胞提取物，使用的抗体是Atg13 (E1Y9V) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Atg13 (hAtg13-Myc/DDK; +), using Atg13 (E1Y9V) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). Western blot 分析293T细胞的细胞提取物，未转染（-）对照或转染了Myc/DDK标签的全长人源的Atg13 (hAtg13-Myc/DDK; +)，使用的抗体是Atg13 (E1Y9V) Rabbit mAb 兔单抗（上图）和Myc-Tag (71D10) Rabbit mAb 兔单抗#2278（下图）。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg13 siRNA I #12043 (+), using Atg13 (E1Y9V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Atg13 (E1Y9V) Rabbit mAb confirms silencing of Atg13 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot 分析HeLa细胞的细胞提取物，分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，或 SignalSilence® Atg13 siRNA I #12043 (+)，使用抗体是Atg13 (E1Y9V) Rabbit mAb 兔单抗(上图) 或者 β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下图)。The Atg13 (E1Y9V) Rabbit mAb 兔单抗证实Atg13表达的沉默，使用β-Actin (D6A8) Rabbit mAb兔单抗作为上样的对照。 | |
Immunoprecipitation of Atg13 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (lane 2) or Atg13 (E1Y9V) Rabbit mAb (lane 3). Lane 1 is 10% input. 对MCF7细胞提取物中的Atg13进行免疫共沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 Atg13 (E1Y9V) Rabbit mAb 兔单抗 (泳道3)。泳道1为10%的上样。Western blot 分析使用抗体是Atg13 (E1Y9V) Rabbit mAb 兔单抗。 |
