| 货号 | 7613S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Atg4A (D62C10) Rabbit mAb recognizes endogenous levels of total Atg4A protein. This antibody recognizes unidentified bands within the molecular weight range of 48-60 kDa which decrease with silencing of Atg4A expression. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg) (1). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes (2). Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologues (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4) (3-5). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue (6). |
| 存放说明 | -20C |
| 计算分子量 | 48-60 |
Western blot analysis of extracts from various cell lines using Atg4A (D62C10) Rabbit mAb.Western blot分析多种细胞系的细胞提取物，使用抗体是Atg4A (D62C10) Rabbit mAb。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a DYKDDDDK-tagged human Atg4A construct (+), using Atg4A (D62C10) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).Western blot分析293T细胞的细胞提取物，对照转染(-)或转染DYKDDDDK标签的人源 Atg4A 质粒(+)，使用抗体是Atg4A (D62C10) Rabbit mAb（上图）和DYKDDDDK Tag Antibody #2368 (下图). | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg4A siRNA I #6427 (+), using Atg4A (D62C10) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Atg4A (D62C10) Rabbit mAb confirms silencing of Atg4A expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western blot分析HeLa细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® Atg4A siRNA I #6427 (+)，使用抗体是Atg4A (D62C10) Rabbit mAb (上图) 或 α-Tubulin (11H10) Rabbit mAb #2125 (下图)。Atg4A (D62C10) Rabbit mAb证明Atg4A的表达被沉默，α-Tubulin (11H10) Rabbit mAb用来检测内参。 |
