| 货号 | 8558S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Atg7 (D12B11) Rabbit mAb recognizes endogenous levels of total Atg7 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from various cell lines using Atg7 (D12B11) Rabbit mAb. Western blot 分析不同细胞系的提取物，使用的抗体是Atg7 (D12B11) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Atg7 siRNA I #6604 (+), using Atg7 (D12B11) Rabbit mAb (upper) or α-Tubulin (11Η10) Rabbit mAb #2125 (lower). The Atg7 (D12B11) Rabbit mAb confirms silencing of Atg7 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 分析HeLa细胞的提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® Atg7 siRNA I #6604 (+)转染，使用的抗体是Atg7 (D12B11) Rabbit mAb 兔单抗（上图）或 α-Tubulin (11Η10) Rabbit mAb 兔单抗#2125 （下图）。在以 α-Tubulin (11H10) Rabbit mAb 兔单抗作为加样对照，Atg7 (D12B11) Rabbit mAb 兔单抗证实Atg7表达的沉默。 |
