| 货号 | 2439S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | ATGL (30A4) Rabbit mAb detects endogenous levels of total ATGL protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
Western blot analysis of extracts from NIH/3T3 and differentiated NIH/3T3-L1 cells, using ATGL (30A4) Rabbit mAb. 对NIH/3T3和分化的3T3-L1细胞裂解液使用ATGL (30A4) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse lung, showing staining specific to adipocytes, using ATGL (30A4) Rabbit mAb. 对石蜡包埋的小鼠肺使用ATGL (30A4) Rabbit mAb兔单抗进行免疫组化分析，显示脂肪细胞特异性的染色。 | |
Immunohistochemical analysis of NIH/3T3-L1 cells undifferentiated (left) or differentiated (right), showing induced staining of adipocytes, using ATGL (30A4) Rabbit mAb. 对NIH/3T3-L1细胞，未分化（左）或分化的（右），使用ATGL (30A4) Rabbit mAb兔单抗进行免疫组化分析，显示出诱导的脂肪细胞染色。 | |
Immunohistochemical analysis of paraffin-embedded mouse brown fat, using ATGL (30A4) Rabbit mAb. 对石蜡包埋的小鼠棕色脂肪组织使用ATGL (30A4) Rabbit mAb兔单抗进行免疫组化分析。 | |
Confocal immunofluorescent analysis of 3T3-L1 cells, using ATGL (30A4) Rabbit mAb (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 对3T3-L1细胞使用ATGL (30A4) Rabbit mAb兔单抗（红色）进行共聚焦免疫荧光分析，显示分化细胞的胞质定位。脂质小滴被BODIPY 493/503 (绿色)标记。蓝色伪彩=DRAQ5?(荧光DNA染料)。 |
